抗骨质疏松药物对双足鼠脊柱侧弯模型的影响△

Effect of two anti-osteoporosis drugs on scoliosis progression in bipedal mice

摘要:

[目的] 探讨阿仑膦酸钠 (alendronate, AL) 和特立帕肽 (teriparatide, TPTD) 对双足鼠脊柱侧弯进展的作用。[方法] 将 45 只 C57BL/6J 雌性 4 周龄小鼠行双前肢和尾离断，建立双足鼠脊柱侧弯模型，随机分为 3 组，每组 15 只。对照组、 AL 组和 TPTD 组，分别给予生理盐水、AL 和 TPTD，连续 12 周。行影像和骨密度 (bone mineral density, BMD) 检测。[结果] 随时间推移，三组动物 Cobb 角均显著增加 (P＜0.05)。术后 8 周和 12 周，脊柱侧弯 Cobb 角由大至小依次为：对照组＞AL 组＞ TPTD 组，总体差异有统计学意义 (P＜0.05)；两两比较，对照组的 Cobb 角显著大于 AL 组和 TPTD 组 (P＜0.05)，而 AL 组与 TPTD 组间差异无统计学意义(P＞0.05)。随时间推移，三组动物 BMD 均显著增加(P＜0.05)。术后 4、8 和 12 周 BMD 值由低至高均为：对照组0.05)。[结论]在双足鼠脊柱侧弯模型早期给予 AL 和 TPTD 可有效减小鼠脊柱侧弯 Cobb 角，同时增加生长期小鼠脊柱 BMD 值。

Abstract:

[Objective] To observe the effects of alendronate (AL) and teriparatide (TPTD) on scoliosis progression in bipedal mice. [Methods] Forty-five 4-week-old female C57BL/6J mice received bilateral forelimb amputation to establish a scoliosis model of bipedal mice. The animals were randomly divided into 3 groups with 15 mice in each group, including the control group, AL group and TPTD group, which were given normal saline, AL and TPTD respectively for 12 weeks. The imaging check and bone mineral density (BMD) assay were conducted. [Results] The Cobb angle increased significantly over time in all the 3 groups (P＜0.05) . At 8 and 12 weeks after amputation, the Cobb angle of scoliosis was ranked in descending order of control group ＞AL group ＞TPTD group with statistically significant overall dif- ference (P＞0.05) , which in the control group was significantly higher than that of AL group and TPTD group (P＜0.05) , whereas there was no significant difference between AL group and TPTD group (P＞0.05) . In addition, the BMD significantly increased over time in all three groups (P＜0.05) , which ranked from low to high at 4, 8 and 12 weeks after amputation were as follows: control group 0.05) . [Conclusion] Early administration of AL and TPTD in bipedal mice scoliosis model can effectively reduce Cobb angle and increase spinal BMD in growing mice.