Abstract:[Objective] To describe the relationship between the nerve root and intervertebral space of the upper lumbar spine under unilateral biportal endoscopy, and to provide an evidence for the real surgical treatment of spinal stenosis. [Methods] Radiographic docu- ments were obtained from 21 patients with lumbar spinal stenosis, including lumbar CT myelography, CT three-dimensional reconstruction. From L1/2 to L3/4 segment, the distances were measured including those between the laminar inferior edge-facet medial edge and the laminar superior edge-facet medial edge to the lateral edge of the dural sac, the lower endplate of the upper lumbar spine, the upper endplate of the lower lumbar spine, the lower edge of the nerve root origin, and the facet lateral edge-herringbone crest edge. In addition, the distance be- tween the upper and lower lumbar endplates at the lower edge of the nerve root origin, as well as the distance from the lateral edge of the in- ferior articular process of the upper lumbar spine to the medial edge of the isthmus of the pedicle of the lower lumbar spine were measured. [Results] From L1/2 to L3/4 segment, the laminar inferior edge-facet medial edge and the laminar superior edge-facet medial edge points were within the outer edge of the dural sac, the lower intervertebral space and inferior edge of the nerve root, the smaller the distance (P< 0.05) . The distance from the laminar inferior edge-facet medial edge point and the laminar superior edge-facet medial edge point to the facet lateral edge-herringbone crest edge gradually increased with the segment downward (P<0.05) . The lower edge of the nerve root origin was below the level of intervertebral space, and the lower the segment, the smaller the distance (P<0.05) . The projection of the lateral edge of the inferior articular process of the upper lumbar spine was adjacent to the medial edge of the isthmus of the pedicle of the lower lumbar spine (P>0.05) . [Conclusion] There are obvious anatomical characteristics of the upper lumbar spine. In lumbar spinal stenosis, with larg- er upper and lower decompression range, and the hemi-laminal defect might be induced by decompression of adjacent segments on the same side and slightly larger laminal fenestration, while futher decompression might result in partial defect of inferior articular process and isthmus.