Abstract:[Objective] To introduce the surgical technique and preliminary clinical results of percutaneous lumbar interbody fusion with novel self-developed instrument for lumbar spondylolisthesis. [Methods] From December 2017 to February 2019, 23 patients under- went abovementioned surgical intervention for lumbar spondylolisthesis. Under guide of fluoroscopy, the puncture aiming to the sliding in- tervertebral space was conducted from the entry point 6~9 cm beside the posterior midline, and then the needle was replaced by a guide wire. Foraminoplasty was performed following the guide wire, and the endoscope and instruments were inserted to complete decompression of the lateral recess and intervertebral foramen. The novel self-developed instruments were used to dilate the narrowed intervertebral space in grades. After that, percutaneous pedicle screws and rods were placed to assist reduction of spondylolisthesis. Discectomy with complete remove of endplate cartilage was done with self-developed reamers and curators through working cannel under endoscopic vision, and then bone autograft and a cage were inserted into the space. Finally, the screw-rod system was adjusted to compress the intervertebral space and fastened. [Results] All patients were successfully operated on without serious complications. At the last follow-up, the VAS scores both for back pain and leg pain, ODI score, as well as height of intervertebral space and distance of spondylolisthesis measured on radiographs were significantly improved compared with those before operation (P＜0.05) . CT scanning revealed bony fusion of the affected intervertebral space in all patients at the last interview. [Conclusion] This percutaneous lumbar fusion with the novel self-developed instruments is a safe, ef- fective and minimally invasive surgical method for lumbar spondylolisthesis.