基于Notch通道右归丸对大鼠退变椎间盘的影响
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北京中医药大学第三附属医院

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北京中医药大学第三附属医院2022年度院内培育项目


Investigate the effect of Yougui Wan on degenerative intervertebral disc in rats based on Notch pathway
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1.Beijing University of Chinese Medicine Third Affiliated Hospital;2.北京中医药大学第三附属医院

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    摘要:

    摘要:[目的]探讨右归丸对大鼠退变腰椎间盘结构的潜在干预机制。[方法]将30只雄性SD大鼠随机分为3组,每组10只。正常对照组为健康大鼠,不做特殊处理;退变组采用纤维环穿刺法建立大鼠椎间盘退变模型。药物组在退变组基础上予右归丸灌胃2周。ELISA检测血清炎症因子IL-10、MIF、TNF-α水平;Western blot检测椎间盘组织中Ⅱ型胶原、Notch1蛋白表达水平。[结果]与正常对照组比较,退变组与药物组的大鼠血清炎症因子IL-10、MIF、TNF-α含量增加,Ⅱ型胶原蛋白表达水平明显降低,Notch1蛋白表达水平显著升高,各组差异均具有统计学意义(P<0.05)。与退变组相比,药物组的大鼠血清炎症因子IL-10含量增加,MIF、TNF-α含量减少,Ⅱ型胶原蛋白表达水平升高,Notch1蛋白表达水平降低,各组差异均具有统计学意义(P<0.05)。[结论]右归丸能够抑制椎间盘退变大鼠的炎症反应,减少细胞外基质降解,从而缓解大鼠椎间盘退变,其机制可能与调控Notch信号通路相关。

    Abstract:

    [Objective] To explore the potential intervention mechanism of Yougui Wan on the deformed lumbar intervertebral disc structure in rats. [Methods] A total of 30 male Sprague-Dawley rats were randomly divided into 3 groups, with 10 rats in each group. The animals in the normal control group were healthy rats without special treatment, those in degeneration group were established the intervertebral disc degeneration model by puncturing the annulus. Rats in medical group were gavage Yougui Wan for 2 weeks on the basis of the degeneration group. Serum IL-10,MIF and TNF-α levels were measured by ELISA method, the protein expression levels of CollagenⅡ, Notch1 in intervertebral disc tissues were detected by Western Blot method. [Results] Compared with normal control group, IL-10, MIF and TNF-α levels in degeneration group and medical group were increased, the expression levels of collagenⅡ protein were decreased, and the expression levels of Notch1 protein were increased, the difference among all groups was statistically significant (P<0.05). Compared with the degeneration group, the levels of IL-10 in medical group were increased, the levels of MIF, TNF-α were decreased, the protein expression levels of collagenⅡ were increased, and the protein expression levels of Notch1 were decreased, the difference among all groups was statistically significant (P<0.05). [Conclusion] Yougui Wan can inhibit the inflammatory response of IDD rats, reduce the degradation of extracellular matrix, and alleviate intervertebral disc degeneration. The mechanism may be related to the regulation of Notch signaling pathway.

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  • 收稿日期:2023-08-07
  • 最后修改日期:2023-10-07
  • 录用日期:2023-12-29
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