Abstract:Abstract: Objective To investigate the application significance of internal thread trephine in the biopsy of bone tissue disease. Methods A retrospective analysis was performed on 32 patients with suspected vertebral lesions who underwent bone tissue biopsy in our hospital from January 2021 to December 2022, and they were divided into two groups according to the type of sampling trephination: the observation group (internal thread trephine, 16 cases) and the control group (common trephine, 16 cases). All punctures were performed under local anesthesia. The working channel was routinely established, the trephine was slowly rotated in, fluoroscopic positioning was performed, the trephine was removed after passing through the lesion, and the specimen was removed. According to the clinical data of the patients, the operation-related indexes, test results and postoperative adverse reactions of the two groups were statistically compared. Results There were no differences in age, gender, course of disease and body mass index (BMI) between the two groups (P > 0.05). All patients successfully completed the operation, and the success rate of biopsy in both groups was 100%. The intraoperative blood loss in the observation group and the control group was less than 5ml, and was 1.0~5.0 (2.5±0.7) mL and 1.0~4.8 (2.8±0.9) mL, respectively, with no difference (P > 0.05). The operation time in the observation group and the control group was 9.0~24 (17.1±2.5) min and 11.0~29.0 (19.7±3.1) min, respectively, which was shorter in the observation group (P < 0.05). The sampling segments in the observation group: cervical spine 6 cases, thoracolumbar sacral spine 10 cases, sacroiliac joint 7 cases; The control group: cervical spine 9 cases, thoracolumbar sacral spine 10 cases, sacroiliac joint 12 cases. There were no statistically significant differences in sampling segments, histopathological results, positive rate of puncture tissue (100% vs 93.8%), positive rate of puncture results (87.5% vs 93.8%) and total incidence of postoperative complications (37.5% vs 68.8%) between the two groups (P > 0.05). Conclusions The use of internal thread trephine under negative pressure can significantly shorten the vertebral biopsy time, but has no significant effect on the positive rate of puncture biopsy and the incidence of postoperative complications. Key words: Bone tissue disease biopsy; Internal thread trephine; Common trephine; Operation time; Biopsy positive rate