Abstract:[Objective] To compare the clinical efficacy of three drainage methods after multi-segment transforaminal lumbar interbody fusion (TLIF). [Methods] From July 2019 to June 2020, a total of 104 patients who were undergoing TLIF for multilevel lumbar degenerative diseases in our department were divided into 3 group. After TLIF, 34 patients had intermittent negative pressure drainage (INPD), 37 pa- tients received continuous negative pressure drainage (CNPD), while the remaining 33 patients were treated by natural pressure drainage (NPD). The perioperative and follow-up data of the three groups were compared. [Results] All the patients in the 3 groups had TLIF per- formed smoothly. Although there were no significant differences in operative time and intraoperative blood loss among three groups (P>0.05), the CNPD group proved significantly greater than the INPD and NPD groups in terms of drainage volume at the 1st postoperative day, total vol- ume of postoperative drainage and total drainage days (P<0.05). However, the NPD group had significantly greater incision errhysis than the INPD and CNPD groups (P<0.05). The VAS of leg pain and ODI significantly decreased (P<0.05), while the JOA scores significantly in- creased over time postoperatively in all the 3 groups (P<0.05), whereas which proved not statistically significantly among the 3 groups at any corresponding time points (P>0.05). [Conclusion]The intermittent negative pressure drainage after multilevel TLIF does significantly re- duce the drainage volume, reduce postoperative wound bleeding, and shorten total drainage days, is a safe and effective drainage method.