Abstract:[Objective] To compare the clinical outcomes of discectomy with unilateral biportal endoscopy (UBE) versus uniportal inter- laminar endoscopy (UIE) for single-segment lumbar disc herniation (LDH). [Methods] A retrospective study was conducted on 63 patients who underwent endoscopic discectomy in our department from August 2020 to August 2021. According to the preoperative doctor-patient communication, 32 patients received UBE, while the remaining 31 patients received UIE. The perioperative period, follow-up and imaging data of the two groups were compared. [Results] The UBE group proved significantly poorer than the UIE group in terms of operating time [(83.2±10.3)min vs (71.8±8.9)min, P<0.05], the total length of incision [(1.5±0.2)cm vs (1.2±0.2)cm, P<0.05], intraoperative blood loss [(30.0±2.0)ml vs (20.0±1.6)ml, P<0.05], the ambulation time [(2.5±0.5)days vs (1.6±0.2)days, P<0.05] and hospital stay [(5.1±0.6)days vs (4.0±0.4)days, P<0.05], nevertheless there were no significant differences in intraoperative fluoroscopy times and incision healing grade be- tween the two groups (P>0.05). All of them in both groups were followed up for more than 12 months. VAS scores both for back pain and leg pain, as well as ODI score significantly decreased over time in both groups (P<0.05). The UBE group was significantly better than the UIE group in terms of VAS score for lumbago [(2.2±0.4) vs (2.5±0.5), P<0.05] and VAS score of leg pain [(2.4±0.6) vs (2.8±0.7), P<0.05] and ODI score [(20.2±2.9) vs (22.5±2.5), P<0.05] a month postoperatively, whereas which became not statistically significant between the two groups at 6 months postoperatively and the latest follow-up. Regarding imaging, the spinal canal occupying ratio significantly decreased (P<0.05) and intervertebral height remained unchanged (P>0.05), and the lumbar lordosis increased significantly (P<0.05) in both group at the latest follow- up compared with those preoperatively. By the time of the last follow- up, the UBE group proved significantly superior to the UIE group in terms of canal occupying ratio [(12.3±3.2)% vs (15.5±4.3)%, P<0.05] and lumbar lordosis [(52.3±6.2)° vs (47.8±5.9)°, P<0.05].[Conclusion] Both UBE and UIE are effective in the treatment of LDH, with comparable clinical consequences. By comparison, the UIE is less invasive, while the UBE is more effective decompression.