Abstract:[Objective] To introduce the surgical techniques and preliminary clinical results of electromagnetic navigation guided per- cutaneous pedicle screw for fixation of thoracolumbar burst fractures. [Methods] From January 2020 to January 2022, a total of 30 patients received electromagnetic navigation guided percutaneous pedicle screw for single-segment thoracolumbar burst fractures. After general an- esthesia, the patients were placed in the prone position, and the magnetic field generator, locator and navigation bridge were placed respec- tively. After the C-arm fluoroscopic image was successfully matched with the electromagnetic navigation system, the pedicle screws were percutaneously placed under the guidance of electromagnetic navigation, following by fastened with the bilateral rods that introduced percu- taneously to finish the fixation. [Results] All the patients were successfully operated on without nerve or vascular injury, and followed up for (12.1±2.3) months on a mean. The VAS scores [(0.3±0.1), (6.3±0.3), P＜0.05] and ODI scores [(3.2±0.6), (40.3± 0.8), P＜0.05] significantly reduced postoperatively compared with those preoperatively. In addition, the local kyphotic Cobb angle [(6.4±0.9)° , (23.4±1.6)°, P＜0.05], and compression ratio of the injured vertebra [(3.4±0.5)%, (30.5±2.5)%, P＜0.05] significantly declined at the latest follow-up compared with those preoperatively. During the follow-up period, no broken screw, broken rod, internal fixation loosening and other adverse imaging finding was noted in anyone of them. [Conclusion] This electromagnetic navigation guided percutaneous pedicle screw for thoracolumbar burst fracture does effectively restore vertebral height, significantly reduce symptoms, decrease radiation exposure, and resume spinal stabil- ity well.