Abstract:[Objective] To investigate the clinical efficacy of local injection of autologous plasma and bone marrow concentrate for non- union after internal fixation of tibiofibular fractures. [Methods] From January 2018 to May 2022, a total of 58 patients received local injec- tion of autologous plasma and bone marrow concentrate for nonunion secondary to internal fixation of tibiofibular fractures. The clinical and imaging data were evaluated. [Results] All the 58 patients underwent local injection therapies smoothly without serious adverse reactions, and were followed up for 2 to 8 months. Up to the last follow-up, 56 patients reached the clinical standard of fracture healing, with healing rate of 96.6%, while the remaining 2 patients received open surgeries, including bone grafting for the non-union. From the time point before treatment to 2 months, 4 months, 6 months and 8 months after treatment, the local tenderness VAS scores [(5.1±0.5), (3.8±0.3), (2.9±0.3), (2.5±0.2), (2.0±0.1), P＜0.001], weight-load shearing of the affected limb [(52.0±4.8)%, (59.4±3.5)%, (70.5±3.9)%, (81.4±2.7)%, (93.8± 2.1)%, P＜0.001] and imaging bone callus score [(1.2±0.4), (2.2±0.5), (3.5±0.4), (5.1±0.3), (6.7±0.2), P＜0.001] significantly improved. [Conclusion] Local injection of autologous plasma and bone marrow concentrate is a minimal invasive therapy technique for the nonunion secondary to internal fixation of tibiofibular fractures, with good clinical consequences.