TGF-β1在膝关节骨性关节炎患者中的表达△

Expression of TGF-β1 in serum and local tissues of knee osteoarthritis

摘要:

[目的] 比较膝关节骨性关节炎 （knee osteoarthritis, KOA） 与非 KOA 患者中血清、膝关节软骨和软骨下骨 TGF-β1 （transforming growth factor-β1） 的表达。[方法] 30 例行人工全膝关节置换的 KOA 患者 （KOA 组），另 30 例非 KOA 患者，包括 13 例前交叉韧带急性损伤行韧带重建及髁间窝成形术的患者为对照组。取血清和膝关节软骨、软骨下骨检测 TGF-β1。[结果]ELISA 法检测患者血清中 TGF-β1 结果，KOA 组（1 929.1±76.8）pg/ml，对照组为（1 611.4±105.9）pg/ml，差异存在统计学意义（P=0.022）。软骨与骨组织 TGF-β1 免疫组织化学染色显示：KOA 组强阳性 24 例，阴性 6 例，阳性率为 80.0%；对照组阳性 4 例，阴性 9，阳性率为 30.8%。KOA 组患者膝关节组织中的 TGF-β1 免疫组织化学染色的阳性率高于对照组，差异存在统计学意义（P=0.002）。[结论]与非 KOA 的患者相比，KOA 患者的血清和膝关节相关组织中 TGF-β1 表达显著升高。

Abstract:

[Objective] To compare the expression of transforming growth factor-β1 (TGF-β1) in serum and in knee cartilage and subchondral bone in knee osteoarthritis (KOA) and non-KOA patients. [Methods] Thirty patients with KOA who underwent total knee replacement (the KOA group) and another 30 patients without KOA, including 13 patients with acute anterior cruciate ligament injury who underwent ligament reconstruction and intercondylar fossaplasty (the control group) had TGF-β1 detected in serum, knee cartilage and subchondral bone. [Results] The results of TGF-β1 in serum by ELISA were (1 929.1±76.8) pg/ml in the KOA group, while (1 611.4±105.9) pg/ml in the control group, with a statistically significant difference (P=0.022). Immunohistochemical staining of TGF-β1 in cartilage and bone tissues showed that there were 24 strong positive cases and 6 negative cases in KOA group with a positive rate of 80.0%, whereas 4 positive cases and 9 negative cases with the positive rate of 30.8% in the control group, and the positive rate of TGF-β1 immunohistochemical staining in KOA group was higher than that in control group (P=0.002). [Conclusion] Compared with non-KOA patients, TGF-β1 expression in serum and knee joint related tissues was significantly increased in the KOA patients.