Abstract:[Objective] To evaluate the safety and clinical efficacy of unilateral biportal endoscopy (UBE) for sequestrated lumbar disc herniation (SLDH). [Methods] A retrospective study was conducted on 27 patients who received endoscopic discectomy of SLDH from July 2020 to October 2021. All the patients had the prolapsed free nucleus pulposus tissue removed by UBE under general anesthesia in prone position, and the clinical and imaging results were evaluated. [Results] All the 27 patients were operated successfully, with the average operation time of (88.5±12.8) min, with no vascular, nerve injury, spinal hyperbaric reaction and other related complications occurred during the operation, despite of dural tear in a case. As time went from that preoperative to 6 months postoperatively and the last follow-up, which was lasted for the average of (19.2±4.0) months, the VAS score for the low back pain [(3.2±1.8), (0.8±0.5), (0.2±0.2), P<0.001], VAS score for leg pain [(7.8±1.1), (1.0±0.4), (0.4±0.4), P<0.001] and ODI scores [(74.4±5.3), (17.8±1.3), (10.7±1.3), P<0.001] significantly reduced, whereas the JOA score significantly increased [(14.5±2.7), (22.6±3.1), (25.9±2.8), P<0.001]. At the last follow-up, only one patient got recurrence, and the excellent rate of clinical outcome was 96.3% based on the modified MacNa's criteria. As for imaging, the spinal canal occupied rate decreased significantly over time [(56.4±13.3)%, (8.9±3.0)%, (8.9±3.0)%, P<0.001], while the disc height and lumbar lordotic angle of the involved level remained unchanged significantly (P>0.05). [Conclusion] The unilateral biportal endoscopy is safe and effective in the treatment of sequestrated lumbar disc herniation, and achieves satisfactory clinical outcomes.