小鼠同种异体皮质骨锚钉植入肱骨头实验（开放获取）

(Open Access) Allogenic cortical bone anchor implantation into humeral head in mice

摘要:

[目的] 将小鼠同种异体皮质骨锚钉植入肱骨头，观察检测成骨愈合情况。[方法] 54 只C57BL6/J 小鼠随机分为两组，27 只用于制备同种异体皮质骨锚钉；另外27 只为同种异体皮质骨锚钉植入受体。于植入术后1、2、4、6、8、10 周各有3 只动物行组织观察、免疫组化检测。1、4、10 周时各有3 只动物行Mcro-CT 检测。[结果] 组织形态方面，同种异体皮质骨锚钉植入后，同种异体皮质骨锚钉周围骨小梁逐渐增加，同种异体皮质骨锚钉逐渐被吸收，新生骨小梁长入同种异体皮质骨锚钉内部。Micro-CT 检测显示，随着1 周、4 周和10 周的时间推移，骨小梁体积显著增加[(0.20±0.37) mm3, (0.27±0.35) mm3, (0.34±0.38) mm3, P=0.001]。免疫组化检测显示，随植入后1、2、4、6、8 和10 周的时间推移，OCN [(0.48±0.05), (0.65±0.05), (0.64±0.06), (0.68±0.11), (0.73±0.03), (0.72±0.03), P=0.004]、TGF-β1 [(0.49±0.02), (0.58±0.02), (0.64±0.02), (0.67±0.01), (0.72±0.01), (1.07±0.07), P＜0.001]、JNK1 [(0.51±0.02), (0.63±0.01), (0.65±0.01), (0.68±0.07), (0.71±0.10), (0.83±0.19), P=0.022] 的光密度（optical densi-ty, OD） 值均显著升高。[结论] 小鼠同种异体皮质骨锚钉植入肱骨头可验证材料的生物学性能，证实同种异体皮质骨锚钉在宿主骨内成骨愈合能力良好。

Abstract:

[Objective] To observe and detect the osteogenic capacity of allogenic cortical bone anchors implanted into the humeralhead in mice. [Methods] A total of 54 C57BL6/J mice were randomly divided into two groups, 27 mice were used for preparing allogenic cor-tical bone anchors, while another 27 mice were used as receptors for allograft cortical bone anchors. Three animals were sacrificed for tissueobservation and immunohistochemical detection at 1 week, 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks after implantation, additional-ly, for Mcro-CT at 1, 4 and 10 weeks respectively. [Results] In term of histological observation, trabecular bone gradually increased aroundallogenic cortical bone anchors after implantation, while the allogenic cortical bone anchor was gradually absorbed, with new bone growinginto the interior of allogenic cortical bone anchors. Micro-CT showed that the trabecular volume increased significantly with the time of 1, 4and 10 weeks [(0.20±0.37) mm3, (0.27±0.35) mm3, (0.34±0.38) mm3, P=0.001]. Immunohistochemical assays showed that with time of 1, 2, 4,6, 8 and 10 weeks after implantation, osteocalcin (OCN) [(0.48±0.05), (0.65±0.05), (0.64±0.06), (0.68±0.11), (0.73±.03), (0.72±0.03), P=0.004], TGF-β1 [(0.49±0.02), (0.58±0.02), (0.64±0.02), (0.67±0.01), (0.72±0.01), (1.07±0.07), P＜0.001], andc-Jun N-terminal kinase(1JNK1) [(0.51±0.02), (0.63±0.01), (0.65±0.01), (0.68±0.07), (0.71±0.10), (0.83±0.19), P=0.022] significantly increased in term of opticaldensity (OD) values. [Conclusion]The biological properties of mouse allogenic cortical bone anchors can be verified by implantation into hu-merus head, and the osteogenic capacity of the allogenic cortical bone anchors in host bone is proved to be good.