二代测序在假体周围感染诊断应用的进展△（开放获取）

(Open Access) Progress of second generation sequencing in the diagnosis of periprosthetic joint infection

摘要:

假体周围感染是关节置换术后的一种严重并发症，需要大量的医疗资源，给患者和社会带来了巨大的经济负担。近年来，随着新型分子诊断技术的不断发展，假体周围感染的诊断和治疗取得了重大进展。与传统培养相比，二代测序可以快速、准确、全面地诊断致病微生物，提高假体周围感染中致病微生物的检出率，从而实现早期诊断和治疗假体周围感染。本文综述了二代测序的原理、方法和其在假体周围感染中识别致病微生物的特点、优势和局限性。

Abstract:

Periprosthetic joint infection (PJI) is a serious complication after joint replacement, which requires a lot of medical resourcesand brings a huge economic burden to patients and society. In recent years, with the continuous development of novel molecular diagnostictechniques, the diagnosis and treatment of PJI have made great progress. Compared with traditional culture, the next-generation sequencingcan diagnose pathogenic microorganisms quickly, accurately and comprehensively, and improve the detection rate of pathogenic microorgan-isms in PJI, so as to achieve early diagnosis and treatment of PJI. This paper reviews the principle and method of next-generation sequencingand its characteristics, advantages and limitations in identifying pathogenic microorganisms in PJI.