Abstract:[Objective] To compare the efficiency of trephines with or without inner thread for spinal bony tissue biopsy. [Methods] Aretrospective study was conducted on 96 patients who underwent spinal bony tissue biopsy from January 2020 to August 2023. Based on pre-operative surgeon-patient discussion, 50 patients had biopsy performed by trephine with inner thread (the IT group), while the remaining 46patients were by trephine of non-IT ( the non-IT group). The perioperative items, test results and postoperative adverse reactions were com-pared between the two groups. [Results] Although there were no significant differences in biopsy segment, incision length, intraoperativeblood loss and biopsy success rate between the two groups (P>0.05), the IT group proved significantly superior to the non-IT group in termsof operative time [(17.0±2.8) min vs (19.5±3.0) min, P<0.001], and the positive rate of tissue harvesting (87.8% vs 64.5%, P<0.001), in addi-tion, the former was significantly better than the latter regarding to the overall incidence of postoperative complications (20.0% vs 54.4%, P<0.001). [Conclusion] Using the trephine with inner thread does significantly shorten the spine biopsy time, increase the positive rate of punc-ture biopsy, and reduce the incidence of postoperative complications.