蛇葡萄根提取物对大鼠慢性骨髓炎愈合的影响△

Effect ofradix smpelopsis sinicae extract on healing process of chronic osteomyelitis in rats

摘要:

[目的]探讨蛇葡萄根提取物对慢性骨髓炎反应的影响。[方法]将大鼠分为5组，分别为正常组、模型组、低剂量、中剂量和高剂量。每组6只。模型组、低剂量、中剂量和高剂量大鼠接受骨髓炎造模手术。正常组和模型组大鼠每天灌服 1次蒸馏水（10ml）；低剂量、中剂量、高剂量组大鼠每天灌服1次含蛇葡萄根提取物250μg/ml、500μg/ml、1000μg/ml。给药8周后处死大鼠，检测NF-κB信号通路关键基因和炎症因子表达水平，X线片评价大鼠骨髓炎疾病严重程度。[结果]RT qPCR检测，与正常组比较，模型组IL-6、IL-1β、TNF-αmRNA水显著增高（P＜0.05）；与模型组比较，给药的三组上述指标均显著降低（P＜0.05）。与正常组比较，模型组NF-κB、IKK、PU1、FOL水平显著增高（P＜0.05），IκB水平显著降低（P＜0.05）； 与模型组相比，低剂量、中剂量和高剂量NF-κB[(1.8±0.0)vs(1.7±0.1)vs(1.4±0.1)vs(1.3±0.1),P＜0.001]，IKK[(2.3±0.1)vs(2.0±0.1) vs(1.6±0.1)vs(1.3±0.0),P＜0.001]、PU1[(2.6±0.1)vs(1.9±0.1)vs(1.7±0.1)vs(1.4±0.1),P＜0.001]、FOLR[(2.1±0.1)vs(1.8±0.1)vs(1.5± 0.1)vs(1.4±0.1),P＜0.001]水平指标均显著降低，而IκB水平[(0.4±0.0)vs(0.5±0.1)vs(0.6±0.1)vs(0.8±0.0),P＜0.001]显著升高，并呈剂量依赖性。Westernblot检测，与正常组比较，模型组IL-6、IL-1β、TNF-α水平显著增高（P＜0.05），与模型组比较，三个给药组上述指标均显著降低（P＜0.05）。[结论]蛇葡萄根提取物可以抑制NF-κB信号通路关键基因和炎症因子表达，降低炎性细胞浸润，对大鼠骨髓炎具有一定治疗作用。

Abstract:

[Objective] To investigate the effect of radix smpelopsis sinicae extract on the healing process of chronic osteomyelitis in rat.

[Methods] The rats were divided into 5 groups, including normal group, model group, low dose, medium dose and high dose, with 6 animals

in each group. Of them, the rats in model group, low dose, medium dose and high dose rats underwent osteomyelitis model making operation.

Normal group and model group were given distilled water (10 ml) once a day, whereas low dose, medium dose and high dose groups were giv-

en 250 μg/ml, 500 μg/ml and 1 000 μg/ml of radix smpelopsis sinicae extract respectively once a day. After 8 weeks of administration, the

rats were killed, and the expression levels of key genes and inflammatory factors in NF-κB signaling pathway were detected, while the severi-

ty of osteomyelitis was evaluated by X-ray and histology. [Results] As for RT-qPCR assay, the model group had significant increases in

mRNA levels of IL-6, IL-1β and TNF-α compared with the normal group (P<0.05); however, the three drug-given groups got significantly

decreases regarding abovesaid items compared with the model group (P<0.05). In addition, the model group had significant increases in the

levels of NF-κB, IKK, PU1 and FOL, while significant decline of IB compared with the normal group (P<0.05). Compared with the model

group, the low dose, middle dose and high dose groups got significant decrease in terms the nf-kappa B [(1.8±0.0) vs (1.7±0.1) vs (1.4±0.1) vs

(1.3±0.1), P<0.001]. IKK [(2.3±0.1) vs (2.0±0.1) vs (1.6±0.1) vs (1.3±0.0), P<0.001], PU1 [(2.6±0.1) vs (1.9±0.1) vs (1.7±0.1) vs (1.4±0.1), P<

0.001], FOLR [(2.1±0.1) vs (1.8±0.1) vs (1.5±0.1) vs (1.4±0.1), P<0.001], whereas significant upgrading in IB level [(0.4±0.0) vs (0.5±0.1) vs

(0.6±0.1) vs (0.8±0.0), P<0.001] in dosage-dependent manner. Regarding western blot analysis, the model group had significant increase in IL-6, IL-1β and TNF-α compared with the normal group (P<0.05), while the above indexes were significantly decreased in the three drug

administration groups compared with the model group (P<0.05). [Conclusion] The radix smpelopsis sinicaeextract might inhibit the expres-

sion of key genes of NF-κB signaling pathway and inflammatory factors, reduce inflammatory cell infiltration, and has a certain therapeutic

effect on osteomyelitis in rats.