Abstract:[Objective] To compare the morphological differences in lumbar pedicle between isthmic spondylolisthesis and simple lum-bar spinal stenosis, and analyze its clinical significance. [Methods] From January 2020 to October 2023, 62 patients with L5 isthmic spon-dylolisthesis (the IS group) and 45 patients with simple L5S1 spinal stenosis (the SS group) who underwent posterior pedicle screw internalfixation in our hospital were included in this study. There were 31 cases of grade I and 31 cases of grade II. The CT parameters were com-pared between the IS group and the SS group as well as the spondylolisthesis subgroups. [Results] From L1 to L5, the IS group proved signifi-cantly greater than the SS group in terms of pedicle angle (PA) [(12.4±2.8)° vs (8.9±2.7)°, P<0.001; (13.4±2.7)° vs (10.2±2.7)°, P<0.001;(18.2±3.9)° vs (14.6±3.4)°, P<0.001; (22.3±4.8)° vs (17.2±4.5)°, P<0.001; (31.5±4.7)° vs (28.3±4.7)°, P=0.002], and pedicle length (PL)[(18.3±1.6) mm vs (17.4±1.6) mm, P=0.008; (17.1±1.3) mm vs (16.3±1.7) mm, P=0.020; (16.9±1.7) mm vs (16.1±1.7) mm, P=0.029; (14.7±1.4) mm vs (14.1±1.4) mm, P=0.018; (15.8±1.5) mm vs (14.3±2.1) mm, P<0.001]. In addition, the IS group had significantly greater screwtrajectory length (STL) than the SS group (P<0.05), whereas the former had significantly less pedicle width (PW) than the latter from L1 toL5 levels (P<0.05). However, there was no significant difference in all CT parameters from L1 to L5 levels between degree I and degree II ISsubgroups (P>0.05). [Conclusion] The isthmic spondylolisthesis presents the lumbar pedicle change of thinning and lengthening, whichmay be related to the pathogenesis. The inward angle of pedicle screw placement should appropriately be increased.