Abstract:[Objective] To evaluate the significance of magnetic resonance imaging (MRI) examination at 90° knee flexion for the diagnosis of old posterior cruciate ligament (PCL) rupture. [Methods] A retrospective study was conducted on 32 patients who had unilateral PCL tear treated surgically in our hospital from January 2018 to December 2023. All patients were screened for PCL injury by drawer test, underwent MRI examination in extension position and 90° knee flexion, and got old PCL rupture confirmed by arthroscopic surgery. The MRI images were evaluated by two radiologists and a consensus was reached. The MRI measurments were compared and analyzed with those of arthroscopic surgery. [Results] The MRI at 90° knee flexion showed that the normal PCL was of (43.6±1.2) mm in length, (7.5±0.8) in mm width and (77.9±1.2)° in sagittal angle, while the old torn PCL was of (46.8±2.5) mm in length, (9.4±0.9) mm in width and (74.3± 1.3)° in sagittal angle. By comparison, the MRI at knee extension measured the old torn PCL (37.3±1.1) mm in length, (10.1±0.9) mm in width and (70.2±1.4)° in sagittal angle. There were statistical significant differences in the old torn PCL in width, length and sagittal angle between the knee extension and 90° knee flexion position (P<0.001). The length variation ratio between the normal PCL and torn PCL increased from 3.6% at knee extension position to 7.3% at 90° knee flexion, while the length variation ratio between the knee extension and 90° knee flexion was 21.1% for normal PCL, while 25.5% for the old torn PCL. [Conclusion] MRI examination at 90° knee flexion can significantly expand the length variation ratio of the PCL, which is conducive to improving the accuracy of diagnosis of old PCL injury.