红景天苷对大鼠急性脊髓损伤的影响△（开放获取）

Effects of salidroside on acute spinal cord injury in rats

摘要:

[目的]探讨红景天苷对大鼠急性脊髓损伤组织水通道蛋白 1（aquaporin 1, AQP-1）和水通道蛋白 4（AQP-4）表达的影响。[方法] 48 只大鼠被分为假手术组、模型组和红景天苷组，每组 16 只；假手术组仅行椎板切除术，术后给予生理盐水腹腔注射；模型组和红景天苷组制备脊髓损伤模型，术后分别给予生理盐水和红景天苷腹腔注射；术后 3 d 取材，检测脊髓组织含水率，进行尼氏染色、免疫组化、Tuenl 染色、Western blot、RT-PCR 检测。[结果]与假手术组相比，模型组和红景天苷组脊髓组织含水率显著增高，与模型组相比，红景天苷组脊髓组织含水率显著降低 [(85.3±5.6)% vs (72.2±7.6)%, P＜0.001]；尼氏染色显示，假手术组神经细胞形态正常，模型组神经细胞形态紊乱，红景天苷组神经细胞形态较模型组显著改善；免疫组化显示，与假手术组相比，模型组和红景天苷组 NeuN 阳性表达显著下降 ，与模型组相比，红景天苷组 NeuN 阳性表达显著增高 [(0.4±0.4 ) vs (0.9±0.4), P＜0.001]；Tunel 染色显示，与假手术组相比，模型组和红景天苷组 Tunel 阳性染色显著增高，与模型组相比，红景天苷组 Tunel 阳性染色显著降低 [(0.8±0.1) vs (0.6±0.0), P＜0.001]。Western blot 及 RT-PCR 显示，与假手术组相比， 模型组和红景天苷组 AQP-1 和 AQP-4 mRNA 蛋白相对表达量显著增高 （P＜0.05），与模型组相比，红景天苷组 AQP-1 和 AQP-4 mRNA 蛋白相对表达量显著降低（P＜0.05）。[结论] 红景天苷可抑制急性脊髓损伤过程中 AQP-1 和 AQP-4 表达，减轻水肿并抑制细胞凋亡。

Abstract:

[Objective] To investigate the effects of salidroside on the spinal cord tissue expressions of aquaporin 1 (AQP-1) and aquaporin 4 (AQP-4) after acute spinal cord injury in rats. [Methods] Thirty-six rats were randomly divided into the sham operation group, the model group and the salidroside group, with 12 rats in each group. In the sham operation group, only laminectomy was performed, and normal saline was injected intraperitoneally after the operation. In the model group and the salidroside group, spinal cord injury models were established, and normal saline and salidroside were injected intraperitoneally after the operation, respectively. Samples were harvested 3 days after the operation to detect the moisture content of spinal cord tissues. In addition, Nissl, immunohistochemistry and TUNEL staining, as well as western blot and RT-PCR assay were carried out for detection. [Results] Compared with the sham operation group, the moisture contents of spinal cord tissues in the model group and the salidroside group were significantly increased, while compared with the model group, the moisture content of spinal cord tissues in the salidroside group was significantly decreased [(85.3±5.6)% vs (72.2±7.6)%, P＜ 0.001]. In term of Nissl staining, the morphology of nerve cells in the sham operation group was normal, while that in the model group was disordered, and that in the salidroside group was significantly improved compared with the model group. In term of immunohistochemistry, compared with the sham operation group, the positive expressions of NeuN in the model group and the salidroside group were significantly decreased, whereas compared with the model group, the positive expression of NeuN in the salidroside group was significantly increased [(0.4±0.4) vs (0.9±0.4), P＜0.001]. In term of Tunel staining, compared with the sham operation group, the positive Tunel staining in the model group and the salidroside group was significantly increased, while compared with the model group, the positive staining in the salidroside group was significantly decreased [(0.8±0.1) vs (0.6±0.0), P＜0.001]. In term of western blot assay, compared with the sham operation group, the relative protein expression levels of AQP-1 and AQP-4 in the model group and the salidroside group were significantly increased (P＜0.05), while compared with the model group, the AQP-1 and AQP-4 expression in the salidroside group were significantly decreased (P＜ 0.05). As results of RT-PCR, compared with the sham operation group, the relative mRNA expression of AQP-1 and AQP-4 in the model group and the salidroside group were significantly increased (P＜0.05), whereas compared with the model group, the AQP-1 and AQP-4 expression in the salidroside group were significantly decreased (P＜0.05). [Conclusion] The salidroside down- regulates the expressions of AQP-1 and AQP-4 during the process of acute spinal cord injury, reduce edema and inhibit cell apoptosis.