长链非编码RNA lnc02126在尤文氏肉瘤中的表达及与预后的关系△

Expression of lncRNA 02126 in Ewing ’s sarcoma and its relationship with prognosis

摘要:

[目的] 探讨长链非编码 RNA 02126 (lncRNA02126) 对尤文氏肉瘤患者预后的影响及其潜在的作用机制。[方法] 从国际癌症基因组联合体 (International Cancer Genome Consortium, ICGC) 数据库中获取了 56 例尤文氏肉瘤患者的基因表达数据。Kaplan-Meier 生存分析和单多因素分析探究 lnc02126 与尤文氏肉瘤患者预后之间的关系，RT-qPCR 检测 lnc02126 在尤文肉瘤细胞中的表达水平。通过 GO 和 KEGG 分析了解 lnc02126 在尤文氏肉瘤的生物学进展中可能参与的信号通路，通过 CMap 数据库(Connectivity Map)预测对尤文氏肉瘤具有潜在治疗作用的药物[结果]lnc02126 高表达组的尤文氏肉瘤患者预后明显较差 (P=0.025)。lnc02126 可作为一个独立的预后风险因素 (P＜0.001)。RT-qPCR 实验结果表明 lnc02126 在尤文氏肉瘤细胞中显著高表达 (P=0.0043)。GO 和 KEGG 分析发现细胞外的结构组织构建、细胞黏附、血管生成等相关信号通路是 lnc02126 引起尤文氏肉瘤恶性进程的分子机制。CMap 分析显示石蒜碱、伊洛前列素、毒胡萝卜素对尤文氏肉瘤具有潜在治疗作用。[结论]lnc02126 是尤文氏肉瘤的独立预后分子标志物，有望成为尤文氏肉瘤的治疗靶点。

Abstract:

[Objective] To investigate the effect of long noncoding RNA 02126 (lncRNA02126) on the prognosis of Ewing’s sarcoma and its potential mechanism. [Methods] The gene expression data of 56 Ewing’s sarcoma patients were obtained from ICGC (International Cancer Genome Consortium) database. Kaplan-Meier survival analysis and univariate analysis were used to explore the relationship be- tween lnc02126 and prognosis of Ewing’s sarcoma. RT-qPCR was used to detect the expression of lnc02126 in Ewing’s sarcoma cells. Go and KEGG analysis were used to understand the possible signal pathway of lnc02126 in the biological progress of Ewing’s sarcoma. CMAP (connectivity map) predicted the potential therapeutic drugs for Ewing’s sarcoma. [Results] The prognosis of Ewing’s sarcoma patients with high expression of lnc02126 was significantly poor (P=0.025) . lnc02126 was an independent prognostic risk factor (P＜0.001) . RT-qPCR results showed that lnc02126 was highly expressed in Ewing sarcoma cells (P＜0.05) . Go and KEGG analysis showed that the molecular mechanism of malignant progression of Ewing’s sarcoma induced by lnc02126 was related to the signal pathways of extracellular structure, tissue construction, cell adhesion and angiogenesis. CMap analysis showed that lycorine, iloprost and thapsigargin had potential therapeutic effects on Ewing’s sarcoma. [Conclusion] lnc02126 is an independent prognostic molecular marker of Ewing’s sarcoma and may be a thera- peutic target for Ewing’s sarcoma.