Abstract:[Objective] To evaluate the significance of ultrasound-guided selective nerve root block (SNRB) in determining the responsi- ble segment of multi-level lumbar degenerative lesions. [Methods] From September 2019 to August 2021, 72 patients received SNRB to identify the responsible segments of multi-level lumbar degenerative diseases, and then corresponding surgical managements. The docu- ments regarding to SNRB and surgical procedures were evaluated. [Results] Of the 72 patients, 30 (41.7%) got the responsible nerve root lo- cated at the first puncture, 12 (16.7%) were located by the second puncture, and 22 (30.6%) were located by the third time, whereas 8 (11.1%) failed to locate the responsible nerve root, with total positioning success rate of 88.9%. The operation time of SNRB ranged from 20 min to 30 min, with an average of (24.5±2.6)min, while the fluoroscopy performed ranged from 2 times to 5 times, with an average of (3.4±1.0) times. According to the results of imaging localization and SNRB, 12 (16.7% ) underwent single- level spinal canal decompression, 17 (23.6%) received single- level decompression combined with discectomy, 10 (13.9%) were treated with muti-segment decompression, 15 (20.8%) underwent multilevel decompression combined with discectomy, and 18 (25.0%) had decompression combined instrumented fusion performed. All patients had corresponding surgical procedures performed successfully without massive bleeding or nerve injury, and were fol- lowed up for more than 12 months. The VAS scores for lumbago and leg pain, as well as ODI scores significantly decreased (P<0.05), while the JOA scores significantly increased over time (P<0.05). [Conclusion] The SNRB might accurately locate the main responsible segments, narrow the extent of surgical decompression, and improve the efficiency of surgical treatment for multi-level lumbar degenerative diseases.