特发性脊柱侧弯脂联素与软骨细胞增殖△

Adiponectin and chondrocyte proliferation in idiopathic scoliosis

摘要:

[目的] 探讨脂联素是否对特发性脊柱侧弯 (idiopathic scoliosis, IS) 患者关节突软骨细胞的增殖和肥大过程产生影响。[方法]酶联免疫吸附测定法检测 IS 患者和正常人组的血浆脂联素水平；提取 IS 患者和正常人组的关节突原代软骨细胞， 用蛋白质免疫印迹方法检测脂联素及其受体 Adipo R1、软骨细胞标志蛋白 Col Ⅱ和肥大标志物 Col X 的表达情况。不同浓度脂联素刺激 IS 组软骨细胞，检测相关蛋白的表达情况。[结果] IS 患者血浆脂联素水平显著高于正常人组 [(19.3±11.5)μg/ml vs (9.5±8.4)μg/ml, P＜0.05]，且 IS 组女性 [(19.3±11.5)μg/ml vs (11.9±8.8)μg/ml, P＜0.05] 和男性患者 [(9.3±4.5)μg/ml vs (3.7±3.3)μg/ml, P＜ 0.05] 的血浆脂联素水平分别显著高于正常人组女性和男性的水平。IS 患者软骨细胞中脂联素 [(1.4±0.3) vs (1.0±0.1), P＜0.05]、 Adipo R1 [(1.3±0.3) vs (1.0±0.2), P＜0.05] 和 Col Ⅱ [(1.8±0.7) vs (1.0±0.7), P＜0.05] 的蛋白水平显著高于正常人组，而 Col X [(0.2± 0.1) vs (1.0±0.8), P＜0.05] 的蛋白水平则低于正常人组。不同浓度脂联素刺激 IS 组软骨细胞后，Adipo Q、AdipoR1 的表达水平呈浓度依赖性升高 (P＜0.05)，ColⅡ和抗凋亡因子 Bcl2 的蛋白水平也显著升高 (P＜0.05)，而 ColX、Bax 蛋白水平则显著降低 (P＜0.05)。[结论]血浆中升高的脂联素水平可能通过上调 AdipoR1、Bcl2 和 Col Ⅱ，以及下调 Bax 和 Col X 来促进 IS 患者软骨细胞的增殖并抑制其肥大进程。

Abstract:

[Objective] To explore whether adiponectin has an effect on the proliferation and hypertrophy of articular process chondro- cytes in patients with idiopathic scoliosis (IS). [Methods] Plasma adiponectin levels in IS patients and normal subjects were detected by ELISA. Primary chondrocytes of the articular process were isolated from IS patients and normal subjects, and the expressions of adiponectin and its receptor AdipoR1, chondrocyte marker Col Ⅱ and hypertrophy marker ColX were detected by western blotting. Subsequently, the chondrocytes of IS group were stimulated by different concentrations of adiponectin, and the expression of related proteins was detected. [Results] The IS group was measured significantly higher plasma adiponectin than the normal group [(19.3±11.5)μg/ml vs (9.5±8.4)μg/ml, P＜0.05], including the females [(19.3±11.5)μg/ml vs (11.9±8.8)μg/ml, P＜0.05] and the males [(9.3±4.5)μg/ml vs (3.7±3.3)μg/ml, P＜0.05]. In addition, the chondrocytes harvested from IS patients were significantly higher than those of normal control in terms of protein expression of adiponectin [(1.4±0.3) vs (1.0±0.1), P＜0.05], AdipoR1 [(1.3±0.3) vs (1.0±0.2), P＜0.05] and Col Ⅱ [(1.8±0.7) vs (1.0±0.7), P＜0.05], whereas the former had significantly lower Col X than the latter [(0.2±0.1) vs (1.0±0.8), P＜0.05]. The protein expression of Adipo Q and Adi- poR1 in chondrocytes of the IS group increased in a concentration-dependent manner after stimulation with different concentrations of adi- ponectin (P＜0.05), the protein levels of Col Ⅱ and anti-apoptotic factor Bcl2 also significantly increased (P＜0.05), while the protein levels of ColX and Bax significantly decreased (P＜0.05). [Conclusion] Increased adiponectin levels in plasma might promote the proliferation of chondrocytes and inhibit the hypertrophy process in IS patients by upregulating AdipoR1, Bcl2 and Col Ⅱ, and downregulating Bax and Col X.