Abstract:[Objective] To measure the morphological parameters of scapular glenoid on CT data by MIMICS software, and compare the differences between left and right side and between male and female. [Methods] A analysis was conducted on the data of bilateral shoulder CT scan obtained in 84 normal adults from August 2022 to July 2023. The subjects included 45 males and 39 females, aged 18 to 86 years with an average age of (53.8±17.9) years. CT data were imported into MIMICS software for 3D reconstruction, and the concave surface of scapular glenoid, the planes of scapular outline and glenoid outline were determined. Anatomic parameters were measured, including the height, width, height-width ratio, superoinferior (SI) depth, anteroposterior (AP) depth, SI/AP ratio, area of concave glenoid surface (ACGS) and glenoid inclination. [Results] There were no significant differences in height, width, height width ration, SI depth, AP depth, SI/AP depth ratio, ACGS and glenoid inclination between the left and right sides in the 45 adult males and 39 females (P>0.05). However, the male was significantly greater than the female in terms of glenoid height [(37.2±2.4) mm vs (32.6±2.4) mm, P<0.001], width [(28.5±2.8) mm vs (23.2±2.3) mm, P<0.001], and SI depth [(3.6±0.9) mm vs (3.3±1.0) mm, P=0.013], AP depth [(2.0±0.7) mm vs (1.7±0.8) mm, P=0.036], ACGS [(820.6±116.1) mm2 vs (627.9±86.0) mm2 , P<0.001] and absolute value of glenoid inclination [(13.8±7.1)° vs (10.7±4.5)°, P<0.001], whereas the male had significantly less height/width ratio than the female [(1.3±0.1) vs (1.4±0.1), P<0.001], and there was no a significant difference in the SI/AP depth ratio between the two genders [(2.2±0.8) vs (2.3±1.4), P=0.629] on the left side. In addition, totally same anatomic differences were verified on the right side between the male and female. [Conclusion] The MIMICS software used to measure the morphological parameters of scapular glenoid is accurate, quick and visible. The data of this study might provide reference for the evaluation of anatomic abnormality in glenohumeral instability.