Abstract:[Objective] To explore the effect of insert placement sequence in posterior-stabilized total knee arthroplasty (PS-TKA). [Methods] From January 2021 to October 2021, a total 93 patients who were undergoing initial unilateral PS-TKA in our hospital were randomly divided into two groups. Of them, 47 knees had the insert placed firstly, while other 46 knee group had insert placed secondarily. The documents regarding to perioperative period and images of the two groups were compared. [Results] The firstly group proved significantly shorter operation time than the secondarily group [(49.1±3.8) min vs (55.3±4.2) min, P<0.001], althogh there were no significant differences in incision length, intraoperative blood loss, postoperative walking time and incision healing between the two groups (P>0.05). The VAS, HSS scores and knee ROM significantly improved in both groups at discharge compared with those preoperatively (P<0.05). At discharge, the firstly group was significantly better than the secondarily group in term of HSS score [(75.3±5.9) vs (72.2±5.6), P=0.011]. With respect of imaging, the postoperative Hip-knee ankle angle (HKA), medial proximal tibial angle (MPTA) and mechanical lateral distal femoral angle (mLDFA) significantly increased in both groups compared with those preoperatively (P<0.05). Despite of insignificant differences in the above imaging indexes between the two groups before operation (P>0.05), the firstly group got significantly greater mLDFA than the secondarily group postoperatively [(91.1±1.1)° vs (90.6±1.3)°, P=0.011]. [Conclusion] In PS-TKA, the insert placed firstly does reduce the operation steps and shorten the operation time, which is conducive to the recovery of joint function in this study.