Abstract:[Objective] To provide reference for the design of domestic knee femoral prosthesis by CT measurement of the anatomical and morphological parameters of the distal femoral osteotomy surface of the knee in normal Chinese people. [Methods] From March 2022 to October 2022, 187 normal ethnic Han Chinese, including 94 males and 93 females, underwent CT three-dimensional reconstruction of the knee. The mediolateral diameter (ML), anteroposterior diameter (AP), medial condyle width (MCW), lateral condyle width (LCW), width of intercondylar fossa (WICF), and height of intercondylar fossa (HICF) were measured, in addition, the ML/AP ratio were calculated. The gender differences and correlations of each parameter were searched. [Results] The males were measured significantly greater anatomical data of the distal femur than females (P<0.05). Although there was no significant correlation between MCW and WICF (r=0.087, P=0.236), LCW and WICF (r=0.052, P=0.478), HICF and ML/AP (r=0.023, P=0.757), significantly positive correlations among other measurements were noted (P<0.05). The ML/AP of distal femur was negatively correlated with AP (P<0.05), in other word, the ML/AP decreased with the increase of AP. At the same AP value, the male had significantly greater ML/AP than the female (P<0.05). [Conclusion] Chinese men and women have considerable differences in size and shape of distal femoral osteotomy. ML/AP should be considered as a reference in designing femur prostheses with gender differences.