Abstract:[Objective] To introduce the surgical technique and preliminary clinical outcome of 3D printing assisted C1/2 pedicle screw fixation of type II odontoid fracture. [Methods] A total of 14 patients diagnosed with type II odontoid fracture were simulated operation on the customized 3D model and guide before real surgery to determine the size, insertion point and depth of internal fixators. In the real surgery, a posterior median incision was made, lamina and spinous process were exposed after unipolar or bipolar electrocautery. The 3D model was matched with the corresponding real lamina, facet joints and spinous process, screw path was individually drilled along the guide with a fine drill bit. After no error was found, the pedicle screw was inserted and fixed with a titanium rod to finish the fixation. The implant was removed 3 months after the fracture healed firmly. [Results] All the 14 patients got operation performed smoothly without vertebral artery injury, nerve injury or incision infection. Compared with those preoperatively, the VAS score [(7.3±0.8), (2.9±0.6), P<0.001] and NDI score [(61.8±4.9), (32.1±3.0), P<0.001] significantly reduced 6 months postoperatively. Fracture union was achieved in all patients between 9 and 12 months after surgery, and internal fixation was removed around 12 months after surgery. Compared with those before removing internal fixation devices, cervical rotational range of motion (ROM) [(55.8±7.0) °, (85.1±11.9) °, P<0.001] and NDI scores [(25.2±4.9), (10.3± 2.7), P<0.001] significantly improved 7 days after the implant removed. [Conclusion] 3D printing assisted C1/2 pedicle screw fixation of type II odontoid fracture is an effective treatment method, which can significantly restore cervical motion after internal fixation removed.