黄芪多糖对维甲酸诱导骨质疏松大鼠的影响△

Effect of astragalus polysaccharides on osteoporosis induced by retinoic acid in rats

摘要:

[目的] 探究不同浓度的黄芪多糖对维甲酸诱导的骨质疏松大鼠的影响。[方法] 50 只SD 大鼠随机分为对照组、模型组、低剂量组、中剂量组和高剂量组，每组10 只。除对照组外其他各组予以70 mg/kg 维甲酸连续灌肠18 d，对照组使用等体积蒸馏水灌肠。之后，予以黄芪多糖灌胃，低剂量组（100 mg/kg）、中剂量组（200 mg/kg） 和高剂量组（400 mg/kg），对照组和模型组使用等体积蒸馏水，用药第30 d 后，行大鼠左侧股骨大体、BMD、生物力学检测，检测血清TNF-α 和TGF-β1 浓度，检测骨均浆蛋白。[结果] 股骨重量由高至低依次为对照组＞高剂量组＞中剂量组＞低剂量组＞模型组[(1.1±0.2) g vs (1.0±0.1) gvs (0.9±0.2) g vs (0.8±0.1) g vs (0.6±0.0) g, P＜0.001]，对照组与高剂量组间骨重差异无统计学意义（P＞0.05）。BMD 依次为对照组＞高剂量组＞中剂量组＞低剂量组＞模型组[(252.5±21.2) mg/cm2 vs (232.7±21.4) mg/cm2 vs (190.6±31.2) mg/cm2 vs (158.1±13.1) mg/cm2 vs(119.9±12.2) mg/cm2, P＜0.001]，对照组与高剂量组间BMD 差异无统计学意义（P＞0.05）。力学测试的最大载荷排序同上，组间差异有统计学意义（P＜0.05）。血清检测TNF-α 排序为对照组＜高剂量组＜中剂量组＜低剂量组＜模型组，组间差异有统计学意义（P＜0.05）；血清检测TGF-β1 排序为高剂量组＞对照组＞中剂量组＞低剂量组＞模型组，组间差异有统计学意义（P＜0.05）。骨均浆检测BMP-2/β-actin 和p-ERK/ERK 均为对照组＞高剂量组＞中剂量组＞低剂量组＞模型组，组间差异有统计学意义（P＜0.05）。[结论]黄芪多糖可改善维甲酸诱导的大鼠骨质疏松症状，其机理可能是激活MAPK/ERK 信号通路下游。

Abstract:

[Objective] To investigate the effects of different concentrations of astragalus polysaccharide on osteoporosis induced by reti-noic acid in rats. [Methods] Fifty SD rats were randomly divided into control group, model group, low-dose group, medium-dose group andhigh-dose group, with 10 rats in each group. Except the control group, the other groups were given 70 mg/kg retinoic acid continuous intra-gastrically for 18 days, and the control group was given equal volume distilled water. After that, astragalus polysaccharide was given intragas-trically, 100 mg/kg in the low dose group, 200 mg/kg in medium dose group and 400 mg/kg in high dose group, whereas equal volume of dis-tilled water was given in the control group and model group. At 30 days after administration, gross measurement, BMD and biomechanicaltests were performed on the left femur of rats. Serum concentrations of TNF-α and TGF-β1 and bone homogenate protein were measured.[Results] The femur weight in descending order was control group＞high dose group＞medium dose group＞low dose group＞model group [(1.1±0.2) g vs (1.0±0.1) g vs (0.9±0.2) g vs (0.8±0.1) g vs (0.6±0.0) g, P＜0.001], with no significant difference in bone weight between control groupand high-dose group (P＞0.05). BMD was ranked as control group＞high dose group＞medium dose group＞low dose group＞model group[(252.5±21.2) mg/cm2 vs (232.7±21.4) mg/cm2 vs (190.6±31.2) mg/cm2 vs (158.1±13.1) mg/cm2 vs (119.9±12.2) mg/cm2, P＜0.001], and therewas no significant difference in BMD between the control group and the high-dose group (P＞0.05). The order of maximum load in mechani-cal test was the same as above, with statistically significant differences among groups (P＜0.05). The order of serum TNF-α detection was ofcontrol group＜high dose group＜medium dose group＜low dose group＜model group, with statistically significant differences among them (P＜0.05). The order of serum TGF-β1 was of high dose group＞control group＞medium dose group＞low dose group＞model group, with statisticallysignificant differences (P＜0.05). Bone homogenate measured BMP-2/β-actin and P-ERK /ERK were as follows: control group＞high dosegroup ＞ medium dose group＞low dose group＞model group, with statistical significances (P＜0.05). [Conclusion] Astragalus polysaccharidecan improve the osteoporosis induced by retinoic acid in rats, and its mechanism may be the activation of the downstream MAPK/ERK signal-ing pathway.