首页 > 过刊浏览>2024年第32卷第20期 >1875-1881. DOI:10.20184/j.cnki.Issn1005-8478.100782
HTML阅读 XML下载 导出引用 引用提醒
电离辐射诱导椎间盘纤维环细胞衰老的研究△
作者:
作者单位:

1.湖南师范大学附属岳阳医院(岳阳市人民医院) 骨科，湖南岳阳 414000 ;2.美国匹兹堡大学骨科，美国宾夕法尼亚州匹兹堡15213

作者简介:

李佳福，硕士研究生，研究方向：脊柱外科，（电子信箱）17388927834@163.com

中图分类号:

R318

基金项目:

湖南省自然科学基金科药联合项目（编号：2020JJ9054）；湖南省卫生健康委科研课题项目（编号：202104071123）；岳阳市科技基础研究指导性计划项目（编号：202012）


Senescence of annulus fibrosus cells induced by ionizing radiation
Author:
Affiliation:

1.Department of Orthopedics, Yueyang Hospital, Hunan Normal University, Yueyang 414000 , China ;2.Depart⁃ment of Orthopedics, University of Pittsburgh, Pittsburgh, PA15213 , USA

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [22]
    • |
  • 相似文献 [20]
    • | | |
  • 文章评论
    摘要:

    [目的] 探讨电离辐射对大鼠纤维环细胞衰老及衰老相关分泌表型表达的影响。[方法] 选取6 只成熟SD 大鼠，取其腰尾段椎间盘纤维环细胞做体外细胞培养，将同等条件下培养的纤维环细胞随机分为两组，分别接受剂量为0 Gy、15 Gy 的X 线电离辐射，检测两组纤维环细胞的衰老情况以及细胞衰老相关分泌表型（senescence-associated secretory phenotype, SASP）的表达。[结果] 经15 Gy 电离辐射处理后，纤维环细胞在形态学上表现出典型的衰老特征，SA-β-Gal 测定显示纤维环细胞阳性染色百分比为77%。CCK-8 实验显示纤维环细胞9 d 内，未辐射组的OD 值逐渐增加（P＜0.05），而辐射组OD 值比较的差异无统计学意义（P＞0.05）。IF 及PCR 检测均显示，辐射组衰老相关基因，包括P16 [(5.2±0.3) vs (1.0±0.1), P＜0.001]、P21 [(12.9±0.5) vs (1.0±0.1), P＜0.001] 的mRNA 相对表达量均显著高于未辐射组。此外，辐射组SASP 的表达，包括胶原蛋白Col-1 [(1.6±0.1) vs (1.0±0.1), P=0.004]、Col-2 [(7.0±0.4) vs (1.0±0.1), P＜0.001]、白细胞介素（interleukin, IL） -6 [(7.9±0.2) vs (1.0±0.1), P＜0.001]、IL-8 [(1.8±0.1) vs (1.0±0.1), P＜0.001]、基质金属蛋白酶（matrix metalloproteinase, MMP） -1 [(39.2±0.6) vs (1.0±0.1), P＜0.001]、MMP-3 [(4.5±0.3) vs (1.0±0.1), P＜0.001] 的相对表达量均显著高于未辐射组。[结论] 电离辐射能诱导大鼠椎间盘纤维环细胞衰老，其衰老相关分泌表型的表达明显增加。

    Abstract:

    [Objective] To investigate the effects of ionizing radiation on aging and aging-related secretion phenotype expression of ratannulus fibrosus cells. [Methods] Six mature SD rats were selected to harvest lumbar intervertebral disc annulus fibrosus cells. The annu-lus fibrosus cells cultured under the same conditions were randomly divided into two groups, which received ionizing X-ray radiation indose of 0 Gy and 15 Gy respectively. The senescence-associated secretory phenotype (SASP) was assayed to determine the senescence ofannulus fibrosus cells. [Results] After being treated with 15 Gy ionizing radiation, the annulus fibrosus cells showed typical characteristicsof aging morphology, and the positive staining percentage of annulus fibrosus cells was 77% by SA-β-Gal assay. The CCK-8 test showedthat the OD value in the non-irradiated group increased gradually (P＜0.05), but there was no statistical significance in the comparison of OD values in the irradiated group (P＞0.05). Both IF and PCR tests showed that aging related genes in the radiation group, including P16[(5.2±0.3) vs (1.0±0.1), P＜0.001], P21 [(12.9±0.5) vs (1.0±0.1), P＜0.001] were significantly higher than those in the non-radiation group,whereas the expression of SASP in the radiation group including Col-1 [(1.6±0.1) vs (1.0±0.1), P=0.004], Col-2 [(7.0±0.4) vs (1.0±0.1), P＜0.001], IL-6 [(7.9±0.2) vs (1.0±0.1), P＜0.001], IL-8 [(1.8±0.1) vs (1.0±0.1), P＜0.001], matrix metalloproteinase (MMP)-1 [(39.2±0.6) vs(1.0±0.1), P＜0.001] and MMP-3 [(4.5±0.3) vs (1.0±0.1), P＜0.001] were significantly higher than those in the non-radiation group. [Conclu-sion] Ionizing radiation can induce senescence of rat intervertebral disc annulus cells, and the expression of senescence related secretionphenotype is significantly increased.

    参考文献
    [1] 彭兵,杜立龙,张同星,等.椎间盘源性腰痛的发病机制与治疗进展[J].中国矫形外科杂志,2023,31(16):1488-1492.DOI:10.3977/j.issn.1005-8478.2023.16.09.Peng B,Du LL,Zhang TX,et al.Research progress on the mechanism and treatment of discogenic low back pain [J].OrthopedicJournal of China,2023,31(16):1488-1492.DOI:10.3977/j.issn.1005-8478.2023.16.09.
    [2] Hoy D,March L,Brooks P,et al.The global burden of low backpain:estimates from the Global Burden of Disease 2010 study [J].Ann Rheum Dis,2014,3(6):968-974.DOI:10.1136/annrheumdis-2013-204428.
    [3] 万标林,梁伟国.组织工程椎间盘纤维环的研究进展[J].中国矫形外科杂志,2011,19(19):1626-1628.DOI:10.3977/j.issn.1005-8478.2011.19.13.Wan BL,Liang WG.Research progress of tissue-engineered intervertebral disc annulus fibrosus [J].Orthopedic Journal of China,2011,19(19):1626-1628.DOI:10.3977/j.issn.1005-8478.2011.19.13.
    [4] Sato M,Ishihara M,Kikuchi M,et al.The influence of Ho:YAG laser irradiation on intervertebral disc cells [J].Lasers Surg Med,2011,43(9):921-926.DOI:10.1002/lsm.21120.
    [5] 郑敏,Grogan SP,Schaffner T,等.被激光损伤软骨释放的凋亡因子诱导软骨细胞凋亡的研究[J].中华医学杂志,2007,87(21):1496-1500.DOI:10.3760/j:issn:0376-2491.2007.21.015.Zheng M,Grogan SP,Schaffner T,et al.The study of apoptosis factors released from laser injured cartilage inducing apoptosis ofchondrocyte [J].National Medical Journal of China,2007,87(21):1496-1500.DOI:10.3760/j:issn:0376-2491.2007.21.015.
    [6] 金慰芳,朱文菁,王洪复,等.137Csγ 射线照射建立成骨细胞衰老模型的研究[J].中华放射医学与防护杂志,1996,16(3):164-166,218.DOI:10.3760/cma.j.issn.0254-5098.1996.03.110.Jin WF,Zhu WJ,Wang HF,et al.Establishing an ageing model of osteoblasts with 137Csγ-ray irradiation [J].Chinese Journal of Radiological Medicine and Protection,1996,16(3):164-166,218.DOI:10.3760/cma.j.issn.0254-5098.1996.03.110.
    [7] Hong EH,Lee SJ,Kim JS,et al.Ionizing radiation induces cellularsenescence of articular chondrocytes via negative regulation ofSIRT1 by p38 kinase [J].J Biol Chem,2010,285(2):1283-1295.DOI:10.1074/jbc.M109.058628.
    [8] 任洁,汪光明,徐俊.电离辐射诱导神经干细胞衰老的模型研究[J].同济大学学报(医学版),2017,38(2):11-16.DOI:10.16118/j.1008-0392.2017.02.003.Ren J,Wang GM,Xu J.Establishment of ionizing radiation-induced neural stem cell aging mode [J].Journal of Tongji University(Medical Science),2017,38(2):11-16.DOI:10.16118/j.1008-0392.2017.02.003.
    [9] Li M,You L,Xue J,et al.Ionizing radiation-induced cellular senescence in normal,non-transformed cells and the involved DNAdamage response:a mini review [J].Front Pharmacol,2018,9:522.DOI:10.3389/fphar.2018.00522.
    [10] Coppé JP,Patil CK,Rodier F,et al.Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenicRAS and the p53 tumor suppressor [J].PLoS Biol,2008,6(12):2853-2868.DOI:10.1371/journal.pbio.0060301.
    [11] Ngo K,Patil P,McGowan SJ,et al.Senescent intervertebral disccells exhibit perturbed matrix homeostasis phenotype [J].MechAgeing Dev,2017,166:16-23.DOI:10.1016/j.mad.2017.08.007.
    [12] Li X,Li C,Zhang W,et al.Inflammation and aging:signaling pathways and intervention therapies [J].Signal Transduct Target Ther,2023,8(1):239.DOI:10.1038/s41392-023-01502-8.
    [13] Krupkova O,Sadowska A,Kameda T,et al.p38 MAPK facilitatescrosstalk between endoplasmic reticulum stress and IL-6 releasein the intervertebral disc [J].Front Immunol,2018,9:1706.DOI:10.3389/fimmu.2018.01706.
    [14] Sadowska A,Touli E,Hitzl W,et al.Inflammaging in cervical andlumbar degenerated intervertebral discs:analysis of proinflammatory cytokine and TRP channel expression [J].Eur Spine J,2018,27(3):564-577.DOI:10.1007/s00586-017-5360-8.
    [15] Andrade P,Hoogland G,Garcia MA,et al.Elevated IL-1β and IL-6 levels in lumbar herniated discs in patients with sciatic pain [J].Eur Spine J,2013,22(4):714-720.DOI:10.1007/s00586-012-2502-x.
    [16] McGarry T,Biniecka M,Veale DJ,et al.Hypoxia,oxidative stressand inflammation [J].Free Radic Biol Med,2018,125:15-24.DOI:10.1016/j.freeradbiomed.2018.03.042.
    [17] Bedore J,Leask A,Séguin CA.Targeting the extracellular matrix:matricellular proteins regulate cell-extracellular matrix communication within distinct niches of the intervertebral disc [J].MatrixBiol,2014,37:124-130.DOI:10.1016/j.matbio.2014.05.005.
    [18] 龙厚清,陈晓亮,胡有谷.基质金属蛋白酶与腰椎间盘退变的关系[J].中国矫形外科杂志,2000,7(4):393-395.DOI:10.3969/j.issn.1005-8478.2000.04.027.Long HQ,Chen XL,Hu YG.Relationship between matrix metalloproteinases and lumbar disc degeneration [J].Orthopedic Journalof China,2000,7(4):393-395.DOI:10.3969/j.issn.1005-8478.2000.04.027.
    [19] 赵小丹,刘浩,黄富国,等.Ⅰ、Ⅱ型胶原蛋白在不同损伤类型动物模型椎间盘组织内的含量变化规律[J].华西医学,2018,33(9):1146-1152.DOI:10.7507/1002-0179.201807150.Zhao XD,Liu H,Huang FG.Type Ⅰ and Ⅱ collagen in intervertebral discs of animal models with different injury-type changes inthe content of tissues [J].West China Medical Journal,2018,33(9):1146-1152.DOI:10.7507/1002-0179.201807150.
    [20] Chen F,Liu H,Wang X,et al.Melatonin activates autophagy viathe NF-κB signaling pathway to prevent extracellular matrix degeneration in intervertebral disc [J].Osteoarthritis Cartilage,2020,28(8):1121-1132.DOI:10.1016/j.joca.2020.05.011.
    [21] Chaurasia M,Gupta S,Das A,et al.Radiation induces EIF2AK3/PERK and ERN1/IRE1 mediated pro-survival autophagy [J].Autophagy,2019,15(8):1391-1406.DOI:10.1080/15548627.2019.1582973.
    [22] Lv S,Wang X,Jin S,et al.Quercetin mediates TSC2-RHEBmTORpathway to regulate chondrocytes autophagy in knee osteoarthritis [J].Gene,2022,820:146209.DOI:10.1016/j.gene.2022.146209.
    引证文献
    Comments
    Comments
    分享到微博
    Submit
引用本文

李佳福,钟炯彪,Nam Vo,等. 电离辐射诱导椎间盘纤维环细胞衰老的研究△[J]. 中国矫形外科杂志, 2024, 32 (20): 1875-1881. DOI：10.20184/j. cnki. Issn1005-8478.100782.
LI Jia-fu, ZHONG Jiong-biao, Nam Vo, et al. Senescence of annulus fibrosus cells induced by ionizing radiation[J]. Orthopedic Journal of China , 2024, 32 (20): 1875-1881. DOI：10.20184/j. cnki. Issn1005-8478.100782.

复制
文章指标
  • 点击次数:94
  • 下载次数: 267
  • 引用次数: 0
历史
  • 收稿日期:November 01,2023
  • 最后修改日期:May 08,2024
  • 在线发布日期: October 21,2024
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.
Post Code：271000 Tel：0538-6213228
URL：http://jxwk.ijournal.cn/ E-mail：jiaoxingtougao@163.com
Orthopedic Journal of China ® 2025 All Rights Reserved