Abstract: [Objective] To investigate the clinical consequences of 3D printing assisted arthroscopic fixation of anterior cruciate ligament (ACL) tibial avulsion fracture. [Methods] A retrospective study was performed on 58 patients who received arthroscopic fracture reduction and elastic fixation with high-strength suture pulled out through tibial tunnels for ACL tibial avulsion fracture in our hospital from October 2020 to December 2022. According to preoperative doctor-patient communication, 28 patients had simulated surgical operation performed on 3D printed model before the real surgery (the 3D group), while other 30 patients had routinely arthroscopic fixation conducted without preoperative simulation (the routine group). The perioperative, follow-up and imaging data of the two groups were compared. [Results] All patients in both groups had arthroscopic fracture reduction and fixation performed smoothly. The 3D group proved significantly superior to the routine groups in terms of the operation time [(44.6±9.0) min vs (51.3±13.2) min, P=0.029], the postoperative drainage volume [(163.1±29.1) ml vs (180.3±33.4) ml, P=0.019], and the success rate of first establishment of bone tunnel [cases (%), 17 (60.7) vs 10 (33.3), P=0.037]. The follow-up period lasted for (12.7±6.9) months on an average, and the 3D group resumed full weight-bearing activities significantly earlier than that in the routine group [(21.7±5.4) days vs (25.9±6.1) days, P=0.033]. The Lysholm and IKDC scores, as well as anterior drawer test were significantly improved in both groups over time (P<0.05). The 3D group was significantly better than the routine group regarding IKDC score 3 months after surgery [(78.6±6.6) vs (74.1±7.1), P=0.016]. With respect of imaging, the 3D group was also significantly better than the routine group in term of fracture reduction quality [excellent/good/poor, (15/12/1) vs (8/16/6), P=0.045]. The ACL angle was significantly increased (P<0.05), while the anterior ACL crest height was significantly decreased postoperatively compared with those preoperatively in both groups (P<0.05). The 3D group got significantly lower anterior ACL crest height than the routine group 3months postoperatively [(2.3±0.8) mm vs (2.9±0.8) mm, P=0.009]. [Conclusion] Arthroscopic reduction and fixation of ACL tibial avulsion fracture does achieve good clinical outcomes. The 3D printing assistance is helpful to shorten the operation time, improve the success rate of bone tunnel establishment and the quality of fracture reduction, and promote the early recovery of knee joint function.