Abstract:[Objective] To evaluate the efficacy of unilateral biportal endoscopic lumbar interbody fusion (UBE-LIF) in the treatmentof degenerative lumbar diseases. [Methods] A retrospective analysis was conducted on 413 patients who had single-segment degenerativelumbar diseases treated by UBE-LIF treatment at our hospital from 2019 to 2022. The clinical and imaging documents were evaluated. [Re-sults] All the patients had UBE-LIF performed successfully with the average operative time of (158.3±17.2) min, the average intraopera-tive blood loss of (217.5±22.1) ml, and were followed up for a mean of (17.3±3.1) months. With time preoperatively, 6 months after surgeryand at the last follow-up, the low back pain VAS [(6.9±0.6), (1.6±0.3), (1.4±0.2), P<0.001], leg pain VAS [(8.0±0.7), (1.5±0.5), (1.3±0.4),P<0.001] and ODI scores [(78.5±6.1), (17.5±3.7), (17.5±3.7), P<0.001] were significantly declined. In term of imaging, the spinal canal ar-ea [(128.6±19.8) mm2, (238.2±12.5) mm2, (223.4±12.7) mm2, P<0.001], lateral recess sagittal diameter [(1.5±0.5) mm, (6.3±0.5) mm, (6.0±0.5) mm, P<0.001] and intervertebral height [(7.5±0.7) mm, (12.3±0.5) mm, (11.8±0.5) mm, P<0.001] were significantly improved over thetime period abovementioned. The CT showed that the fusion rate was 92.5% 6 months after operation, while the MacNab excellent and goodrate of the clinical consequence was of 94.4% at the last follow-up and the postoperative complication rate was of 4.6%. [Conclusion] Al-though the UBE-LIF consumed longer operation time, it achieves good overall clinical outcome, with low postoperative complication.