Abstract:[Objective] To compare the clinical efficacy of Wiltse approach pedicle screw fixation (WAPSF) versus percutaneous pedi-cle screw fixation (PPSF) in the treatment of single-level thoracolumbar fracture. [Methods] A retrospective study was conducted on 97 pa-tients who received surgical treatment for single-segment thoracolumbar fracture without nerve injury in our department from January 2013to December 2020. According to the preoperative doctor-patient exchange, 51 patients underwent WAPSF, while other 46 patients werewith PPSF. The data of perioperative period, follow-up and imaging were compared between the two groups. [Results] The operation wassuccessfully completed in both groups. The WAPSF group was significantly better than the PPSF group in operation time [(75.5±8.6) min vs(103.5±9.0) min, P<0.001], intraoperative fluoroscopy times [(6.4±0.9) vs (15.4±1.6), P<0.001], the hospitalization cost [(4.3±0.3) 10 k yu-an vs (5.0±0.2) 10 k yuan, P<0.001], but the former had significantly longer total incision length than the latter [(7.0±0.7) cm vs (6.3±0.5)cm, P<0.001]. In addition, the WAPSF group had significantly lower blood creatine kinase (CK) than the PPSF group 1 day after surgery[(155.7±9.9) U/L vs (174.3±15.8) U/L, P<0.001]. The VAS for back pain and ODI scores significantly decreased in both groups with time(P<0.05), and ODI scores in WAPSF group were significantly higher than that in the PPSF group 3 days after surgery (P<0.05). In term ofimaging, there was no significant difference in the accuracy of screw placement between the two groups (P>0.05). The local kyphotic Cobbangle decreased significantly (P<0.05), while the percentage of anterior vertebra height increased significantly in both groups postoperative-ly compared with those preoperatively (P<0.05). The WAPSF group proved significantly superior to the PPSF group regarding the local ky-photic Cobb angle at all time points after operation (P<0.05), and the percentage of anterior vertebral height 3 days after operation (P<0.05).[Conclusion] Both Wiltse approach pedicle screw and percutaneous pedicle screw are minimally invasive fixation of thoracolumbar frac-tures, and do achieve satisfactory clinical consequence. In contrast, the Wiltse approach is less time-consuming, less doctor and patient ra-diation exposure, less hospitalization costs, and more satisfactory in improving thoracolumbar kyphosis angle and restoring the height of the injured vertebrae.