Abstract:[Objective] To evaluate the clinical consequence of 3D printing assisted reduction and fixation of femoral condylar osteochondral fractures in adolescents. [Methods] A retrospective study was conducted on 30 adolescent patients who had femoral condylar osteochondral fractures treated surgically from March 2018 to March 2023. According to the doctor-patient communication, 15 patients received by 3D printing assisted and small-incision reduction and fixation (3D group), while other 15 patients were treated by open reduction and internal fixation (conventional group). Clinical and imaging documents were compared between the two groups. [Results] All patients in both groups had operation performed successfully. The 3D group proved significantly superior to the conventional group in terms of operation time [(64.5±6.1) min vs (75.4±10.0) min, P＜0.001] and incision length [(2.7±0.3) cm vs (3.4±0.4) cm, P＜0.001]. Compared with those preoperatively, the VAS and Kujala scores, as well as knee extension-flexion ROM were significantly improved in both groups at the latest follow-up lasted for more than 1 year (P＜0.05). At the last follow-up, the 3D group was significantly better than the conventional group regarding to the VAS score [(1.1±0.8) vs (1.8±0.8), P=0.031], Kujala scale [(78.5±2.7) vs (75.7±3.0), P=0.012], knee ROM [(124.6±4.7)° vs (120.4 + 4.5)°, P=0.019]. As for imaging, the 3D group was also significantly superior to the conventional group in terms of osteochondral fragment reduction quality [excellent/good/poor, (15/0/0) vs (11/4/0), P=0.032] and healing condition [union/edema/absorption, (14/1/0) vs (9/6/0), P=0.031]. [Conclusion] The 3D printing assisted reduction and fixation of femoral osteochondral fracture do significantly improve knee function, with less surgical trauma and complications.